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This volume of Methods in Molecular Biology provides detailed experimental protocols focused on DNA repair mechanisms in prokaryotic systems. The chapter by A-Lien Lu discusses the repair of A/G and A/8-oxoG mismatches by MutY adenine DNA glycosylase in Escherichia coli. It explains the biochemical activity of MutY, its role in preventing G:C to T:A transversions caused by oxidative damage (8-oxo-7,8-dihydrodeoxyguanine), and describes its dual DNA glycosylase and AP lyase activities. The chapter includes methodology for studying MutY function, enzyme-DNA intermediates, and kinetic properties.

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245: $a: Methods in Molecular Biology, Volume 152: DNA Repair Protocols – Prokaryotic Systems
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100: $a: Pat Vaughan
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